Hotspot

rpoB-trnC-GCA

LSC · 25057-26157 · 1101 bp

Back to Delphinium

Pi 0.0129

MarkerSeek score 70.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 86
pi: 0.0129
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1132-1272 bp	0.961538	81.9
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	TTTCTTGATCAGGCGACACC	50.0	50.0	57.3	58.2	1151-1291 bp	0.961538	81.1
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	58.4	1114-1254 bp	0.961538	79.7
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	1134-1274 bp	0.961538	76.6
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	GACACCCGGATTTGAACTGG	50.0	55.0	57.3	58.8	1137-1277 bp	0.961538	75.8