Hotspot

atpF-atpH

LSC · 11193-11615 · 423 bp

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Pi 0.0145

MarkerSeek score 70.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 40
pi: 0.0145
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CGACCCAAGGAAACGAAAGA	AATTTACGGACTGGTCGTGG	50.0	50.0	58.1	57.9	501-519 bp	1.0	89.0
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	AATTTACGGACTGGTCGTGG	50.0	50.0	58.3	57.9	531-546 bp	0.961538	85.0
atpF-atpH_p3	CGACCCAAGGAAACGAAAGA	ACAATTTACGGACTGGTCGT	50.0	45.0	58.1	57.2	503-521 bp	1.0	83.1
atpF-atpH_p4	CGACCCAAGGAAACGAAAGA	GGTCGTGGCATTAGCACTTT	50.0	50.0	58.1	58.8	489-507 bp	1.0	83.0
atpF-atpH_p5	TTAAATCCGAAACTCCCGGC	ACAATTTACGGACTGGTCGT	50.0	45.0	58.3	57.2	533-548 bp	0.961538	79.1