Hotspot

trnS-GCU-trnG-UCC

LSC · 6430-7127 · 698 bp

Back to Delphinium

Pi 0.0139
MarkerSeek score 72.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
698
variable_sites
59
pi
0.0139
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 792-863 bp 1.0 76.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 791-862 bp 1.0 76.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 793-864 bp 1.0 74.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 792-863 bp 1.0 74.9
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC AACCGAAAGACCCCTTAACT 50.0 45.0 58.4 56.4 794-865 bp 1.0 74.0