Hotspot

rpl2-rpl23

LSC · 89464-89500 · 37 bp

Back to Dehaasia

Pi 0.108

MarkerSeek score 72.60

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 4
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CGGTATAGACGCTTATGGCC	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	729-735 bp	1.0	80.4
rpl2-rpl23_p2	ATAGACGCTTATGGCCTCCT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	725-731 bp	1.0	80.3
rpl2-rpl23_p3	GCTTATGGCCTCCTCCTCTA	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	719-725 bp	1.0	80.3
rpl2-rpl23_p4	TCTGTCCGTGCTTCAAACAA	GAATGGTTAAAGCGCCCAAC	45.0	50.0	58.0	58.0	1168-1174 bp	1.0	80.2
rpl2-rpl23_p5	TTCTGTCCGTGCTTCAAACA	GAATGGTTAAAGCGCCCAAC	45.0	50.0	58.0	58.0	1169-1175 bp	1.0	80.2