Hotspot

petA-psbJ

LSC · 66439-67586 · 1148 bp

Back to Dehaasia

Pi 0.0262

MarkerSeek score 74.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 30
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	45.0	55.0	58.2	58.2	1291-1293 bp	1.0	79.2
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	45.0	55.0	58.2	58.6	1292-1294 bp	1.0	76.2
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	45.0	50.0	58.2	57.1	1290-1292 bp	1.0	73.7
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1284-1286 bp	1.0	73.5
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.1	58.2	1290-1292 bp	1.0	73.1