Hotspot

trnT-GGU-psbD

LSC · 33762-35204 · 1443 bp

Back to Dehaasia

Pi 0.00208

MarkerSeek score 59.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1443
variable_sites: 3
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	55.0	55.0	58.1	57.9	1585 bp	1.0	78.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	50.0	55.0	58.1	57.9	1588 bp	1.0	78.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1593 bp	1.0	77.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1596 bp	1.0	77.5
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	55.0	55.0	58.1	58.3	1586 bp	1.0	77.4