Hotspot

rps16-trnQ-UUG

LSC · 6307-8202 · 1896 bp

Back to Dehaasia

Pi 0.00158

MarkerSeek score 58.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1896
variable_sites: 3
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1969-1978 bp	1.0	78.4
rps16-trnQ-UUG_p2	GGGTACAAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	2002-2011 bp	1.0	77.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1973-1982 bp	1.0	77.3
rps16-trnQ-UUG_p4	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1990-1999 bp	1.0	76.9
rps16-trnQ-UUG_p5	GTACAAGAATCCACAGCCGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	2000-2009 bp	1.0	76.5