Hotspot

matK-trnK-UUU

LSC · 3624-4341 · 718 bp

Back to Dehaasia

Pi 0.00139

MarkerSeek score 59.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTGTTGCCGAAATCCATCCA	GTCGGGCAATCGAATCTCTT	45.0	50.0	58.1	58.1	867 bp	1.0	79.0
matK-trnK-UUU_p2	TGTTGCCGAAATCCATCCAT	GTCGGGCAATCGAATCTCTT	45.0	50.0	57.9	58.1	866 bp	1.0	78.5
matK-trnK-UUU_p3	TTGTTGCCGAAATCCATCCA	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.1	58.2	790 bp	1.0	78.1
matK-trnK-UUU_p4	TTGTTGCCGAAATCCATCCA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.1	58.2	789 bp	1.0	78.1
matK-trnK-UUU_p5	TTGTTGCCGAAATCCATCCA	ATGGGTTGCTAACTCAACGG	45.0	50.0	58.1	58.2	805 bp	1.0	78.0