Hotspot

ycf1

SSC · 125632-131364 · 5733 bp

Back to Debregeasia

Pi 0.00384

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5733
variable_sites: 33
pi: 0.00384
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTGGATGGGGTGATTTCTTGA	CCAGTAGGTGTTCCGATCAA	47.6	50.0	57.9	56.9	272 bp	1.0	63.8
ycf1_p2	AGTGGATGGGGTGATTTCTTG	CCAGTAGGTGTTCCGATCAA	47.6	50.0	57.9	56.9	273 bp	1.0	63.7
ycf1_p3	GTGGATGGGGTGATTTCTTGA	GCCAGTAGGTGTTCCGATC	47.6	57.9	57.9	57.6	273 bp	1.0	61.3
ycf1_p4	AGTGGATGGGGTGATTTCTTG	GCCAGTAGGTGTTCCGATC	47.6	57.9	57.9	57.6	274 bp	1.0	61.2
ycf1_p5	GTGGATGGGGTGATTTCTTGA	GCCAGTAGGTGTTCCGATCA	47.6	55.0	57.9	59.5	273 bp	1.0	60.5