Hotspot

rpl16

LSC · 82521-83954 · 1434 bp

Back to Debregeasia

Pi 0.0014
MarkerSeek score 60.40
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1434
variable_sites
3
pi
0.0014
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GCAAAAGGCGACGTAAAAA TGTCTTTTAACTCAAAACAGAA 42.1 27.3 55.3 52.1 1619-1627 bp 1.0 40.7
rpl16_p2 GCAAAAGGCGACGTAAAAA TTGTCTTTTAACTCAAAACAGA 42.1 27.3 55.3 52.1 1620-1628 bp 1.0 40.7
rpl16_p3 TTCGTCCGAAAAACCTTTTT TGTCTTTTAACTCAAAACAGAA 35.0 27.3 54.3 52.1 1548-1556 bp 1.0 40.7
rpl16_p4 TTCGTCCGAAAAACCTTTTT TTGTCTTTTAACTCAAAACAGA 35.0 27.3 54.3 52.1 1549-1557 bp 1.0 40.7
rpl16_p5 TTTCGTCCGAAAAACCTTTT TGTCTTTTAACTCAAAACAGAA 35.0 27.3 54.3 52.1 1549-1557 bp 1.0 40.7