Hotspot

ccsA

SSC · 115886-116842 · 957 bp

Back to Debregeasia

Pi 0.00279

MarkerSeek score 33.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 957
variable_sites: 4
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	ATGTCATCAAGCGAGAACCC	50.0	50.0	58.0	58.0	1252 bp	1.0	80.1
ccsA_p2	TGAAATCGGTAGACACGCTG	ATGTCATCAAGCGAGAACCC	50.0	50.0	58.0	58.0	1251 bp	1.0	80.1
ccsA_p3	GTGAAATCGGTAGACACGCT	AACCGGGTATGTCATCAAGC	50.0	50.0	58.0	58.0	1260 bp	1.0	80.0
ccsA_p4	TGAAATCGGTAGACACGCTG	AACCGGGTATGTCATCAAGC	50.0	50.0	58.0	58.0	1259 bp	1.0	80.0
ccsA_p5	GTGAAATCGGTAGACACGCT	GATTCTGGACCGCGAGAATT	50.0	50.0	58.0	58.1	1524-1525 bp	1.0	79.9