Hotspot

psaJ-rpl33

LSC · 68347-68789 · 443 bp

Back to Debregeasia

Pi 0.00602

MarkerSeek score 61.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 4
pi: 0.00602
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGTTTATTTCCGGATGCGT	ACTCGAGCTTCTTTACCCCT	45.0	50.0	58.0	58.1	507-515 bp	1.0	81.3
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	ACTCGAGCTTCTTTACCCCT	50.0	50.0	58.0	58.1	581-589 bp	1.0	80.9
psaJ-rpl33_p3	TGGCACCGGTAGTAAGTACT	ACTCGAGCTTCTTTACCCCT	50.0	50.0	57.8	58.1	568-576 bp	1.0	79.4
psaJ-rpl33_p4	TCGTTTATTTCCGGATGCGT	CTCGAGCTTCTTTACCCCTG	45.0	55.0	58.0	57.7	506-514 bp	1.0	79.4
psaJ-rpl33_p5	ACATATCTTTCCGTGGCACC	CTCGAGCTTCTTTACCCCTG	50.0	55.0	58.0	57.7	580-588 bp	1.0	78.9