Hotspot

petA-psbJ

LSC · 64054-65024 · 971 bp

Back to Debregeasia

Pi 0.00687

MarkerSeek score 62.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 10
pi: 0.00687
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGTCTTTTGCTCTTCTTGGC	GCCGATACTACTGGAAGGAT	50.0	50.0	57.3	56.2	1193-1201 bp	1.0	60.6
petA-psbJ_p2	GGTCTTTTGCTCTTCTTGGC	GCCGATACTACTGGAAGGATT	50.0	47.6	57.3	57.0	1193-1201 bp	1.0	58.7
petA-psbJ_p3	GGTCTTTTGCTCTTCTTGGC	GCCGATACTACTGGAAGGATTC	50.0	50.0	57.3	58.1	1193-1201 bp	1.0	58.3
petA-psbJ_p4	ACGTGTACAAGGTCTTTTGCT	GCCGATACTACTGGAAGGAT	42.9	50.0	58.1	56.2	1203-1211 bp	1.0	57.6
petA-psbJ_p5	ACGTGTACAAGGTCTTTTGCT	GCCGATACTACTGGAAGGATT	42.9	47.6	58.1	57.0	1203-1211 bp	1.0	55.7