Hotspot

accD-psaI

LSC · 59620-60345 · 726 bp

Back to Debregeasia

Pi 0.00651
MarkerSeek score 66.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
726
variable_sites
7
pi
0.00651
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCTCGAAATCCTTTAAAGGTCG TTGCCGGAAATACTAAGCCC 45.5 50.0 57.1 58.0 850-877 bp 1.0 59.9
accD-psaI_p2 CCTCGAAATCCTTTAAAGGTCG AAACCATAGCAATTGCCGGA 45.5 45.0 57.1 58.2 862-889 bp 1.0 59.0
accD-psaI_p3 CCTCGAAATCCTTTAAAGGTCG AACCATAGCAATTGCCGGAA 45.5 45.0 57.1 58.2 861-888 bp 1.0 59.0
accD-psaI_p4 CCTCGAAATCCTTTAAAGGTCG ACCATAGCAATTGCCGGAAA 45.5 45.0 57.1 58.2 860-887 bp 1.0 59.0
accD-psaI_p5 CCTCGAAATCCTTTAAAGGTCG GAAACCATAGCAATTGCCGG 45.5 50.0 57.1 57.8 863-890 bp 1.0 58.6