Hotspot

rpoB-trnC-GCA

LSC · 26867-28032 · 1166 bp

Back to Debregeasia

Pi 0.00572

MarkerSeek score 60.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 10
pi: 0.00572
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCCGATCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1304-1321 bp	1.0	78.3
rpoB-trnC-GCA_p2	GCCCCGATCAATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.4	58.1	1301-1318 bp	1.0	77.8
rpoB-trnC-GCA_p3	TAAGCCCCGATCAATGAACC	TTGTTAATCAGGCGACACCC	50.0	50.0	57.7	58.2	1322-1339 bp	1.0	77.5
rpoB-trnC-GCA_p4	GTTCTTCTGTTAAGCCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1314-1331 bp	1.0	77.3
rpoB-trnC-GCA_p5	AGTTCTTCTGTTAAGCCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1315-1332 bp	1.0	77.2