Hotspot

trnK-UUU-rps16

LSC · 4350-5168 · 819 bp

Back to Debregeasia

Pi 0.00244

MarkerSeek score 62.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 3
pi: 0.00244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	898-917 bp	1.0	80.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	897-916 bp	1.0	80.5
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.3	58.0	907-926 bp	1.0	79.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGTGTTTT	50.0	45.0	58.2	58.2	894-913 bp	1.0	78.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGTGTTT	50.0	45.0	58.2	58.2	895-914 bp	1.0	78.7