Hotspot

petB

Genome · 70428-71884 · 1457 bp

Back to Davallia

Pi 0.102

MarkerSeek score 81.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1457
variable_sites: 145
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCCTCAAAGGGGTCTCTGAG	GTCACACACTCCCGTAATCC	55.0	55.0	58.1	58.0	2230-2243 bp	1.0	88.5
petB_p2	CACCAAAACTCAGAACGGGA	GTCACACACTCCCGTAATCC	50.0	55.0	58.0	58.0	1733 bp	0.5	59.6
petB_p3	TCACCAAAACTCAGAACGGG	GTCACACACTCCCGTAATCC	50.0	55.0	58.0	58.0	1734 bp	0.5	59.6
petB_p4	CACCAAAACTCAGAACGGGA	GAGGACGAACACGCAAAAAG	50.0	50.0	58.0	58.0	2253 bp	0.5	59.5
petB_p5	TCACCAAAACTCAGAACGGG	GAGGACGAACACGCAAAAAG	50.0	50.0	58.0	58.0	2254 bp	0.5	59.5