Hotspot

ndhB

Genome · 1-1224 · 1224 bp

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Pi 0.211

MarkerSeek score 55.60

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 1224
variable_sites: 8
pi: 0.211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.03
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	TCTTGGGGCGACTTATCAAA	ACCAATTTTGCTTTCTCGGA	45.0	40.0	56.8	55.9	1179 bp	0.5	33.5
ndhB_p2	TGGGGCGACTTATCAAATTGA	ACCAATTTTGCTTTCTCGGA	42.9	40.0	57.6	55.9	1176 bp	0.5	31.9
ndhB_p3	ATCTTGGGGCGACTTATCAA	ACCAATTTTGCTTTCTCGGA	45.0	40.0	56.6	55.9	1180 bp	0.5	31.6
ndhB_p4	TCTTGGGGCGACTTATCAAA	ACCAATTTTGCTTTCTCGGAT	45.0	38.1	56.8	56.4	1179 bp	0.5	30.0
ndhB_p5	TCTTGGGGCGACTTATCAAAT	ACCAATTTTGCTTTCTCGGA	42.9	40.0	57.4	55.9	1179 bp	0.5	29.6