Hotspot

trnL-UAG-ccsA

Genome · 111907-112181 · 275 bp

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Pi 0.292

MarkerSeek score 73.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 275
variable_sites: 80
pi: 0.292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	AGCCCTTCTCCGTCTATACC	ACCAATGAGACTGCAAAGCA	55.0	45.0	58.0	58.0	1211 bp	0.5	59.9
trnL-UAG-ccsA_p2	GCCCTTCTCCGTCTATACCT	ACCAATGAGACTGCAAAGCA	55.0	45.0	58.0	58.0	1210 bp	0.5	59.9
trnL-UAG-ccsA_p3	AGCCCTTCTCCGTCTATACC	AAGGAAACATCGACGACTCG	55.0	50.0	58.0	58.0	1040 bp	0.5	59.8
trnL-UAG-ccsA_p4	GCCCTTCTCCGTCTATACCT	AAGGAAACATCGACGACTCG	55.0	50.0	58.0	58.0	1039 bp	0.5	59.8
trnL-UAG-ccsA_p5	GCCCTTCTCCGTCTATACCT	AAGCAGGTACTAATGGCGTG	55.0	50.0	58.0	58.0	1195 bp	0.5	59.8