Hotspot

rpl20-rps12

Genome · 64265-65110 · 846 bp

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Pi 0.122

MarkerSeek score 84.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 846
variable_sites: 99
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AAGAATCCTATCGAGGCGGA	GCGTTATGGAGGCAGAAGAA	50.0	50.0	58.0	58.0	2115 bp	0.5	59.8
rpl20-rps12_p2	AATTCCATTTTTCCGCGCTG	GCGTTATGGAGGCAGAAGAA	45.0	50.0	58.0	58.0	1628 bp	0.5	59.8
rpl20-rps12_p3	CGAGTGTCTTGCGATTCAGA	GCGTTATGGAGGCAGAAGAA	50.0	50.0	58.0	58.0	1692 bp	0.5	59.7
rpl20-rps12_p4	AATCCGAGCAATCCACAGAC	GCGTTATGGAGGCAGAAGAA	50.0	50.0	58.0	58.0	1601 bp	0.5	59.6
rpl20-rps12_p5	CAGTGAGCAGGGAAGAATCC	GCGTTATGGAGGCAGAAGAA	55.0	50.0	58.0	58.0	2127 bp	0.5	59.5