Hotspot

trnT-GGU-trnfM-CAU

Genome · 35696-36196 · 501 bp

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Pi 0.203

MarkerSeek score 83.20

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 501
variable_sites: 96
pi: 0.203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	TTAGCCCCTTGTCGGATTTG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.9	58.2	574-599 bp	1.0	89.6
trnT-GGU-trnfM-CAU_p2	GTTAGCCCCTTGTCGGATTT	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.9	58.2	575-600 bp	1.0	89.5
trnT-GGU-trnfM-CAU_p3	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.7	58.2	549-574 bp	1.0	88.4
trnT-GGU-trnfM-CAU_p4	CGACTTACGCCTTACCATGG	CAGGTTCAAATCCTGTCCCC	55.0	55.0	58.4	58.2	548-573 bp	1.0	87.3
trnT-GGU-trnfM-CAU_p5	CGTCACTCTACCCCTGAGTT	CAGGTTCAAATCCTGTCCCC	55.0	55.0	58.5	58.2	528-553 bp	1.0	87.1