Hotspot

psbD-trnT-GGU

Genome · 34264-35623 · 1360 bp

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Pi 0.17
MarkerSeek score 85.70
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1360
variable_sites
219
pi
0.17
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 TTTTCCGATGGCAATGGTCA AACTCAGGGGTAGAGTGACG 45.0 55.0 58.1 58.5 1442-1445 bp 1.0 87.5
psbD-trnT-GGU_p2 TTTCCGATGGCAATGGTCAT AACTCAGGGGTAGAGTGACG 45.0 55.0 57.9 58.5 1441-1444 bp 1.0 87.1
psbD-trnT-GGU_p3 TTTTCCGATGGCAATGGTCA TGATTTGAGGCCCCCTTAAC 45.0 50.0 58.1 57.5 1459-1462 bp 1.0 86.9
psbD-trnT-GGU_p4 TTTTCCGATGGCAATGGTCA TTTGAGGCCCCCTTAACTCA 45.0 50.0 58.1 58.5 1456-1459 bp 1.0 86.7
psbD-trnT-GGU_p5 TTTCCGATGGCAATGGTCAT TGATTTGAGGCCCCCTTAAC 45.0 50.0 57.9 57.5 1458-1461 bp 1.0 86.4