Hotspot

trnC-GCA-trnG-GCC

Genome · 30008-30536 · 529 bp

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Pi 0.207

MarkerSeek score 82.90

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 529
variable_sites: 95
pi: 0.207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	526-589 bp	1.0	81.7
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	50.0	57.9	57.9	57.8	528-591 bp	1.0	81.5
trnC-GCA-trnG-GCC_p3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	527-590 bp	1.0	81.5
trnC-GCA-trnG-GCC_p4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.2	58.2	514-577 bp	1.0	81.1
trnC-GCA-trnG-GCC_p5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	50.0	57.9	58.2	57.8	515-578 bp	1.0	80.9