Hotspot

psbM-petN

Genome · 28232-29398 · 1167 bp

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Pi 0.202

MarkerSeek score 83.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1167
variable_sites: 223
pi: 0.202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TTCTCGCGTTTGTAGCTACC	AAGACGGAGGACTGCAAATC	50.0	50.0	58.0	57.9	1819-1849 bp	1.0	90.2
psbM-petN_p2	TTCTCGCGTTTGTAGCTACC	TGTTGTTTCTGCCCGAATCA	50.0	45.0	58.0	58.0	1919 bp	0.5	59.7
psbM-petN_p3	TTCTCGCGTTTGTAGCTACC	TTTATTGCGCCAAGATCGGA	50.0	45.0	58.0	58.0	1692 bp	0.5	59.6
psbM-petN_p4	TTCTCGCGTTTGTAGCTACC	CCTTTGAATAGGGAGCCGTC	50.0	55.0	58.0	58.0	1546 bp	0.5	59.5
psbM-petN_p5	TTCTCGCGTTTGTAGCTACC	TTTCATGTGTCTCGATGCGT	50.0	45.0	58.0	57.9	1631 bp	0.5	59.3