Hotspot

rpoB-trnD-GUC

Genome · 26703-27385 · 683 bp

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Pi 0.231

MarkerSeek score 84.00

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 683
variable_sites: 148
pi: 0.231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	CGAGCGGTTTTGTCGAAAAA	55.0	45.0	58.0	57.9	1412 bp	0.5	59.2
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	CGAGCGGTTTTGTCGAAAAA	55.0	45.0	58.0	57.9	1413 bp	0.5	59.2
rpoB-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	GACGCACCCTTTTCAGGTTA	55.0	50.0	58.0	58.1	1211 bp	0.5	59.0
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	GACGCACCCTTTTCAGGTTA	55.0	50.0	58.0	58.1	1212 bp	0.5	59.0
rpoB-trnD-GUC_p5	GATATCCGCGTCTGTAGCAG	CGAGCGGTTTTGTCGAAAAA	55.0	45.0	58.0	57.9	2077 bp	0.5	59.0