Hotspot

atpH-atpI

Genome · 13678-14425 · 748 bp

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Pi 0.165

MarkerSeek score 83.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 748
variable_sites: 111
pi: 0.165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TTCTGGCTGTCTCGCAATAC	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1460 bp	0.5	59.9
atpH-atpI_p2	TTCTGGCTGTCTCGCAATAC	TCGTCATCGCCTTGTTAGTG	50.0	50.0	58.0	58.0	1494 bp	0.5	59.8
atpH-atpI_p3	AATTACTTGAGGGAGCAGCG	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1981 bp	0.5	59.8
atpH-atpI_p4	AATTGAAGCGAGGCCTACAG	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1394 bp	0.5	59.8
atpH-atpI_p5	GGGAAAGGAGGGGTAGAGTT	AGGTGCCAGAAATCTGCAAA	55.0	45.0	58.0	58.0	1623 bp	0.5	59.7