primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ndhB	1	TCTTGGGGCGACTTATCAAA	ACCAATTTTGCTTTCTCGGA	20	20	45.0	40.0	56.8	55.9	0.0	0.0	3.312985	189	1188	1179-1179	0.500000	33.5
ndhB_p2	ndhB	2	TGGGGCGACTTATCAAATTGA	ACCAATTTTGCTTTCTCGGA	21	20	42.9	40.0	57.6	55.9	0.0	0.0	3.508058	189	1188	1176-1176	0.500000	31.9
ndhB_p3	ndhB	3	ATCTTGGGGCGACTTATCAA	ACCAATTTTGCTTTCTCGGA	20	20	45.0	40.0	56.6	55.9	0.0	0.0	3.544980	189	1188	1180-1180	0.500000	31.6
ndhB_p4	ndhB	4	TCTTGGGGCGACTTATCAAA	ACCAATTTTGCTTTCTCGGAT	20	21	45.0	38.1	56.8	56.4	0.0	0.0	3.755309	189	1188	1179-1179	0.500000	30.0
ndhB_p5	ndhB	5	TCTTGGGGCGACTTATCAAAT	ACCAATTTTGCTTTCTCGGA	21	20	42.9	40.0	57.4	55.9	0.0	0.0	3.794914	189	1188	1179-1179	0.500000	29.6
rps16_p1	rps16	1	GCCTTCCCCTCCTTTTTCAA	GACTTCTGAAGCTGAGTCGG	20	20	50.0	55.0	58.0	58.0	0.0	17.0	0.020251	3630	4681	1592-1592	0.500000	59.8
rps16_p2	rps16	2	GCCTTCCCCTCCTTTTTCAA	GGACTTCTGAAGCTGAGTCG	20	20	50.0	55.0	58.0	58.0	0.0	17.0	0.020251	3630	4681	1593-1593	0.500000	59.8
rps16_p3	rps16	3	GCCTTCCCCTCCTTTTTCAA	AGGGGATAAGCACCAAGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037094	3630	4681	1641-1641	0.500000	59.7
rps16_p4	rps16	4	GCCTTCCCCTCCTTTTTCAA	CTTTGTGAGGGGGAAAACCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090335	3630	4681	1553-1553	0.500000	59.3
rps16_p5	rps16	5	GCCTTCCCCTCCTTTTTCAA	TTGTGAGGGGGAAAACCAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090335	3630	4681	1551-1551	0.500000	59.3
psbK-psbI_p1	psbK-psbI	1	GCCGCAGTTAGCTTTCGATA	AGACAAATAGTTTGAGGGTAAGCA	20	24	50.0	37.5	58.4	58.1	0.0	9.2	4.556139	7205	7757	555-599	1.000000	55.6
psbK-psbI_p2	psbK-psbI	2	GCCGCAGTTAGCTTTCGATA	TGCATAGACAAATAGTTTGAGGGT	20	24	50.0	37.5	58.4	58.2	0.0	9.2	4.616915	7205	7757	560-604	1.000000	55.1
psbK-psbI_p3	psbK-psbI	3	GCCGCAGTTAGCTTTCGATA	GACAAATAGTTTGAGGGTAAGCA	20	23	50.0	39.1	58.4	56.8	0.0	9.2	4.644681	7205	7757	554-598	1.000000	54.8
psbK-psbI_p4	psbK-psbI	4	TAGCTTTTGTTTGGCAAGCC	AGACAAATAGTTTGAGGGTAAGCA	20	24	45.0	37.5	57.5	58.1	11.7	9.2	4.648211	7205	7757	572-616	1.000000	54.8
psbK-psbI_p5	psbK-psbI	5	TAGCTTTTGTTTGGCAAGCC	TGCATAGACAAATAGTTTGAGGGT	20	24	45.0	37.5	57.5	58.2	11.7	9.2	4.708988	7205	7757	577-621	1.000000	54.3
atpH-atpI_p1	atpH-atpI	1	TTCTGGCTGTCTCGCAATAC	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	4.9	0.010497	13678	14425	1460-1460	0.500000	59.9
atpH-atpI_p2	atpH-atpI	2	TTCTGGCTGTCTCGCAATAC	TCGTCATCGCCTTGTTAGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019346	13678	14425	1494-1494	0.500000	59.8
atpH-atpI_p3	atpH-atpI	3	AATTACTTGAGGGAGCAGCG	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	4.9	0.030190	13678	14425	1981-1981	0.500000	59.8
atpH-atpI_p4	atpH-atpI	4	AATTGAAGCGAGGCCTACAG	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	4.4	4.9	0.030190	13678	14425	1394-1394	0.500000	59.8
atpH-atpI_p5	atpH-atpI	5	GGGAAAGGAGGGGTAGAGTT	AGGTGCCAGAAATCTGCAAA	20	20	55.0	45.0	58.0	58.0	0.0	4.9	0.032074	13678	14425	1623-1623	0.500000	59.7
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	CAAGTTCTGGTAGTACGGGC	CGAGCGGTTTTGTCGAAAAA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.099942	26703	27385	1412-1412	0.500000	59.2
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	GCAAGTTCTGGTAGTACGGG	CGAGCGGTTTTGTCGAAAAA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.099942	26703	27385	1413-1413	0.500000	59.2
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	CAAGTTCTGGTAGTACGGGC	GACGCACCCTTTTCAGGTTA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.119084	26703	27385	1211-1211	0.500000	59.0
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	GCAAGTTCTGGTAGTACGGG	GACGCACCCTTTTCAGGTTA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.119084	26703	27385	1212-1212	0.500000	59.0
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	GATATCCGCGTCTGTAGCAG	CGAGCGGTTTTGTCGAAAAA	20	20	55.0	45.0	58.0	57.9	4.9	0.0	0.123525	26703	27385	2077-2077	0.500000	59.0
psbM-petN_p1	psbM-petN	1	TTCTCGCGTTTGTAGCTACC	AAGACGGAGGACTGCAAATC	20	20	50.0	50.0	58.0	57.9	21.0	0.0	0.113907	28232	29398	1819-1849	1.000000	90.2
psbM-petN_p2	psbM-petN	2	TTCTCGCGTTTGTAGCTACC	TGTTGTTTCTGCCCGAATCA	20	20	50.0	45.0	58.0	58.0	21.0	0.0	0.039037	28232	29398	1919-1919	0.500000	59.7
psbM-petN_p3	psbM-petN	3	TTCTCGCGTTTGTAGCTACC	TTTATTGCGCCAAGATCGGA	20	20	50.0	45.0	58.0	58.0	21.0	4.2	0.044560	28232	29398	1692-1692	0.500000	59.6
psbM-petN_p4	psbM-petN	4	TTCTCGCGTTTGTAGCTACC	CCTTTGAATAGGGAGCCGTC	20	20	50.0	55.0	58.0	58.0	21.0	0.0	0.058375	28232	29398	1546-1546	0.500000	59.5
psbM-petN_p5	psbM-petN	5	TTCTCGCGTTTGTAGCTACC	TTTCATGTGTCTCGATGCGT	20	20	50.0	45.0	58.0	57.9	21.0	0.0	0.092151	28232	29398	1631-1631	0.500000	59.3
trnC-GCA-trnG-GCC_p1	trnC-GCA-trnG-GCC	1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.290119	30008	30536	526-589	1.000000	81.7
trnC-GCA-trnG-GCC_p2	trnC-GCA-trnG-GCC	2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	20	19	50.0	57.9	57.9	57.8	0.0	9.9	1.313054	30008	30536	528-591	1.000000	81.5
trnC-GCA-trnG-GCC_p3	trnC-GCA-trnG-GCC	3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	20	19	50.0	57.9	57.9	57.8	0.0	0.0	1.313054	30008	30536	527-590	1.000000	81.5
trnC-GCA-trnG-GCC_p4	trnC-GCA-trnG-GCC	4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.2	58.2	0.0	0.0	1.364424	30008	30536	514-577	1.000000	81.1
trnC-GCA-trnG-GCC_p5	trnC-GCA-trnG-GCC	5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	20	19	50.0	57.9	58.2	57.8	0.0	0.0	1.387359	30008	30536	515-578	1.000000	80.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.536534	34264	35623	1442-1445	1.000000	87.5
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	57.9	58.5	8.1	0.0	0.595396	34264	35623	1441-1444	1.000000	87.1
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TTTTCCGATGGCAATGGTCA	TGATTTGAGGCCCCCTTAAC	20	20	45.0	50.0	58.1	57.5	8.1	0.0	0.608599	34264	35623	1459-1462	1.000000	86.9
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	TTTTCCGATGGCAATGGTCA	TTTGAGGCCCCCTTAACTCA	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.632045	34264	35623	1456-1459	1.000000	86.7
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TTTCCGATGGCAATGGTCAT	TGATTTGAGGCCCCCTTAAC	20	20	45.0	50.0	57.9	57.5	8.1	0.0	0.667461	34264	35623	1458-1461	1.000000	86.4
trnT-GGU-trnfM-CAU_p1	trnT-GGU-trnfM-CAU	1	TTAGCCCCTTGTCGGATTTG	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.9	58.2	0.0	4.1	0.283360	35696	36196	574-599	1.000000	89.6
trnT-GGU-trnfM-CAU_p2	trnT-GGU-trnfM-CAU	2	GTTAGCCCCTTGTCGGATTT	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.9	58.2	0.0	4.1	0.284854	35696	36196	575-600	1.000000	89.5
trnT-GGU-trnfM-CAU_p3	trnT-GGU-trnfM-CAU	3	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.7	58.2	0.0	4.1	0.449537	35696	36196	549-574	1.000000	88.4
trnT-GGU-trnfM-CAU_p4	trnT-GGU-trnfM-CAU	4	CGACTTACGCCTTACCATGG	CAGGTTCAAATCCTGTCCCC	20	20	55.0	55.0	58.4	58.2	11.2	4.1	0.593072	35696	36196	548-573	1.000000	87.3
trnT-GGU-trnfM-CAU_p5	trnT-GGU-trnfM-CAU	5	CGTCACTCTACCCCTGAGTT	CAGGTTCAAATCCTGTCCCC	20	20	55.0	55.0	58.5	58.2	0.0	4.1	0.613616	35696	36196	528-553	1.000000	87.1
trnL-UAA_p1	trnL-UAA	1	CGACTCGAAACTCTCCCAAG	TGAAAGTTGGGTAGGTTGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.026321	45709	46383	2054-2054	0.500000	59.8
trnL-UAA_p2	trnL-UAA	2	CGACTCGAAACTCTCCCAAG	TAGGTTGGCCCCTACGTAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.066228	45709	46383	2043-2043	0.500000	59.5
trnL-UAA_p3	trnL-UAA	3	CGACTCGAAACTCTCCCAAG	CACTTATTCTCTGACCCGCC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.074703	45709	46383	1930-1930	0.500000	59.4
trnL-UAA_p4	trnL-UAA	4	CGACTCGAAACTCTCCCAAG	GTGACACGGGGATTTTCAGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.123818	45709	46383	1706-1706	0.500000	59.0
trnL-UAA_p5	trnL-UAA	5	TCGAAACTCTCCCAAGTTGC	TGAAAGTTGGGTAGGTTGGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.131341	45709	46383	2050-2050	0.500000	58.9
petA-psbJ_p1	petA-psbJ	1	TTTGAAAAAGTGCAGCTGGC	TGGGGTCGTCTCTTCGATAA	20	20	45.0	50.0	58.1	57.9	17.1	0.0	0.194656	59661	60073	469-727	1.000000	88.3
petA-psbJ_p2	petA-psbJ	2	TTTGAAAAAGTGCAGCTGGC	TTGGGGTCGTCTCTTCGATA	20	20	45.0	50.0	58.1	57.9	17.1	0.0	0.194656	59661	60073	470-728	1.000000	88.3
petA-psbJ_p3	petA-psbJ	3	TTGGCACAGATTTTCCTCGT	TGGGGTCGTCTCTTCGATAA	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.403633	59661	60073	505-763	1.000000	86.5
petA-psbJ_p4	petA-psbJ	4	TTGGCACAGATTTTCCTCGT	TTGGGGTCGTCTCTTCGATA	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.403633	59661	60073	506-764	1.000000	86.5
petA-psbJ_p5	petA-psbJ	5	TGGCACAGATTTTCCTCGTG	TGGGGTCGTCTCTTCGATAA	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.610613	59661	60073	504-762	1.000000	84.9
psbE-petL_p1	psbE-petL	1	GCGTTCTCCTGTATTCCCAG	TCCAGCTAGGGTGATTGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.093261	60841	61588	1085-1102	1.000000	89.4
psbE-petL_p2	psbE-petL	2	TAGCTGATATGGACCCCAGG	TCCAGCTAGGGTGATTGGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.064613	60841	61588	1428-1445	1.000000	89.1
psbE-petL_p3	psbE-petL	3	TAGCTGATATGGACCCCAGG	CCAATTGATCACCGCGTCTA	20	20	55.0	50.0	58.0	58.1	0.0	7.1	0.095439	60841	61588	1474-1491	1.000000	88.8
psbE-petL_p4	psbE-petL	4	CCTATCACAAGTGTACCGGC	TCCAGCTAGGGTGATTGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.098653	60841	61588	1770-1787	1.000000	88.5
psbE-petL_p5	psbE-petL	5	ATATTCGTTTGGGCGAGGAC	TCCAGCTAGGGTGATTGGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.085120	60841	61588	1226-1226	0.500000	59.3
rpl20-rps12_p1	rpl20-rps12	1	AAGAATCCTATCGAGGCGGA	GCGTTATGGAGGCAGAAGAA	20	20	50.0	50.0	58.0	58.0	3.0	0.0	0.027377	64265	65110	2115-2115	0.500000	59.8
rpl20-rps12_p2	rpl20-rps12	2	AATTCCATTTTTCCGCGCTG	GCGTTATGGAGGCAGAAGAA	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.029260	64265	65110	1628-1628	0.500000	59.8
rpl20-rps12_p3	rpl20-rps12	3	CGAGTGTCTTGCGATTCAGA	GCGTTATGGAGGCAGAAGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041563	64265	65110	1692-1692	0.500000	59.7
rpl20-rps12_p4	rpl20-rps12	4	AATCCGAGCAATCCACAGAC	GCGTTATGGAGGCAGAAGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	64265	65110	1601-1601	0.500000	59.6
rpl20-rps12_p5	rpl20-rps12	5	CAGTGAGCAGGGAAGAATCC	GCGTTATGGAGGCAGAAGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.059706	64265	65110	2127-2127	0.500000	59.5
petB_p1	petB	1	TCCTCAAAGGGGTCTCTGAG	GTCACACACTCCCGTAATCC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.061793	70428	71884	2230-2243	1.000000	88.5
petB_p2	petB	2	CACCAAAACTCAGAACGGGA	GTCACACACTCCCGTAATCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044177	70428	71884	1733-1733	0.500000	59.6
petB_p3	petB	3	TCACCAAAACTCAGAACGGG	GTCACACACTCCCGTAATCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044177	70428	71884	1734-1734	0.500000	59.6
petB_p4	petB	4	CACCAAAACTCAGAACGGGA	GAGGACGAACACGCAAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056924	70428	71884	2253-2253	0.500000	59.5
petB_p5	petB	5	TCACCAAAACTCAGAACGGG	GAGGACGAACACGCAAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056924	70428	71884	2254-2254	0.500000	59.5
ycf2-ndhF_p1	ycf2-ndhF	1	CGATTGGTGTTCACGCTAGA	CTATCCTGGTGGAAAAGGGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.067794	106459	107258	1376-1376	0.500000	59.5
ycf2-ndhF_p2	ycf2-ndhF	2	TCGATTGGTGTTCACGCTAG	CTATCCTGGTGGAAAAGGGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.067794	106459	107258	1377-1377	0.500000	59.5
ycf2-ndhF_p3	ycf2-ndhF	3	GAGCCTTATCCTGTTTCCGG	CTATCCTGGTGGAAAAGGGC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.103666	106459	107258	1200-1200	0.500000	59.2
ycf2-ndhF_p4	ycf2-ndhF	4	CCCACTCTATGTTTACGGCC	CTATCCTGGTGGAAAAGGGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.109127	106459	107258	1066-1066	0.500000	59.1
ycf2-ndhF_p5	ycf2-ndhF	5	TGGATGGGGACGCATTAAAG	CTATCCTGGTGGAAAAGGGC	20	20	50.0	55.0	57.9	57.9	3.3	0.0	0.109927	106459	107258	1420-1420	0.500000	59.1
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	AGCCCTTCTCCGTCTATACC	ACCAATGAGACTGCAAAGCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016304	111907	112181	1211-1211	0.500000	59.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GCCCTTCTCCGTCTATACCT	ACCAATGAGACTGCAAAGCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016304	111907	112181	1210-1210	0.500000	59.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AGCCCTTCTCCGTCTATACC	AAGGAAACATCGACGACTCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.023236	111907	112181	1040-1040	0.500000	59.8
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GCCCTTCTCCGTCTATACCT	AAGGAAACATCGACGACTCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.023236	111907	112181	1039-1039	0.500000	59.8
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GCCCTTCTCCGTCTATACCT	AAGCAGGTACTAATGGCGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025953	111907	112181	1195-1195	0.500000	59.8
trnN-GUU-ycf2_p1	trnN-GUU-ycf2	1	GATTTACCAATCCCGCCCTT	GGAATATCCGGCGACTCATC	20	20	50.0	55.0	57.9	58.0	0.0	9.3	0.069696	129788	130305	1711-1711	0.500000	59.4
trnN-GUU-ycf2_p2	trnN-GUU-ycf2	2	GATTTACCAATCCCGCCCTT	TCGCTTTGATTCCTACGTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.071948	129788	130305	1310-1310	0.500000	59.4
trnN-GUU-ycf2_p3	trnN-GUU-ycf2	3	TCGTGTTCATTTCTCCCGAC	GGAATATCCGGCGACTCATC	20	20	50.0	55.0	57.9	58.0	0.0	9.3	0.076878	129788	130305	1654-1654	0.500000	59.4
trnN-GUU-ycf2_p4	trnN-GUU-ycf2	4	TGAATCCCCTGCAACTTTCC	GGAATATCCGGCGACTCATC	20	20	50.0	55.0	58.1	58.0	0.0	9.3	0.078975	129788	130305	1234-1234	0.500000	59.4
trnN-GUU-ycf2_p5	trnN-GUU-ycf2	5	TCGTGTTCATTTCTCCCGAC	TCGCTTTGATTCCTACGTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079130	129788	130305	1253-1253	0.500000	59.4