Hotspot

ycf1

SSC · 125359-130830 · 5472 bp

Back to Daucus

Pi 0.0159
MarkerSeek score 51.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5472
variable_sites
338
pi
0.0159
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTGGCACAAATTCTGGGAA ATGAAGGTGGTATGACCCCA 45.0 50.0 57.6 58.0 643-664 bp 1.0 86.2
ycf1_p2 AAGTGGCACAAATTCTGGGA ATGAAGGTGGTATGACCCCA 45.0 50.0 57.6 58.0 644-665 bp 1.0 86.1
ycf1_p3 CATTTTGGTCCAGGCCTCAA ATGAAGGTGGTATGACCCCA 50.0 50.0 58.4 58.0 422-440 bp 1.0 85.5
ycf1_p4 TGGTCCAGGCCTCAATATCA ATGAAGGTGGTATGACCCCA 50.0 50.0 58.1 58.0 423 bp 0.052632 41.1
ycf1_p5 TGGTCCAGGCCTCAATATCA TGAAGGTGGTATGACCCCAA 50.0 50.0 58.1 58.3 422 bp 0.052632 39.2