Hotspot

ndhF-rpl32

SSC · 113540-114653 · 1114 bp

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Pi 0.0292
MarkerSeek score 70.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1114
variable_sites
119
pi
0.0292
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG TTTAAGGCTGCCCAAGATCC 50.0 50.0 58.0 57.9 1224-1309 bp 1.0 90.6
ndhF-rpl32_p2 AAGCGGGTCGATCAAGTAAC TTTAAGGCTGCCCAAGATCC 50.0 50.0 58.0 57.9 1402-1487 bp 1.0 90.2
ndhF-rpl32_p3 CCGTGTGAACCAAAACCCTA TTTAAGGCTGCCCAAGATCC 50.0 50.0 58.0 57.9 1630-1715 bp 1.0 89.1
ndhF-rpl32_p4 AAGCGGGTCGATCAAGTAAC TCCAGGCTTTTGTTGGTTGA 50.0 45.0 58.0 57.8 1801-2081 bp 0.631579 75.3
ndhF-rpl32_p5 CCGTGTGAACCAAAACCCTA TCCAGGCTTTTGTTGGTTGA 50.0 45.0 58.0 57.8 2029-2309 bp 0.631579 74.5