Hotspot

petA-psbJ

LSC · 62594-63585 · 992 bp

Back to Daucus

Pi 0.0259
MarkerSeek score 78.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
992
variable_sites
108
pi
0.0259
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTTACGTGTCCAAGGCCTTT GCTGGTATTCTTGTGATCGGT 50.0 47.6 58.1 58.1 1128-1196 bp 1.0 81.9
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC GCTGGTATTCTTGTGATCGGT 50.0 47.6 57.8 58.1 1115-1183 bp 1.0 81.6
petA-psbJ_p3 CTTACGTGTCCAAGGCCTTT AGCTGGTATTCTTGTGATCGG 50.0 47.6 58.1 57.8 1129-1197 bp 1.0 81.1
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC AGCTGGTATTCTTGTGATCGG 50.0 47.6 57.8 57.8 1116-1184 bp 1.0 80.8
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT GCTGGTATTCTTGTGATCGGT 45.0 47.6 57.6 58.1 1127-1195 bp 1.0 79.9