Hotspot

ycf3-trnS-GGA

LSC · 44268-45144 · 877 bp

Back to Daucus

Pi 0.0228
MarkerSeek score 69.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
877
variable_sites
74
pi
0.0228
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 47.6 45.0 58.0 57.8 785-949 bp 1.0 80.6
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 47.6 50.0 58.0 58.3 787-951 bp 1.0 80.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA CAGTTCCAATGCTACGCCTT 47.6 50.0 58.0 58.5 788-952 bp 1.0 77.7
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA GTTCCAATGCTACGCCTTCA 47.6 50.0 58.0 58.6 786-950 bp 1.0 77.7
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA TCCAATGCTACGCCTTCAAC 47.6 50.0 58.0 58.6 784-948 bp 1.0 77.7