Hotspot

trnE-UUC-trnT-GGU

LSC · 30913-31076 · 164 bp

Back to Daucus

Pi 0.121
MarkerSeek score 65.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
164
variable_sites
66
pi
0.121
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CTGACTATCGTGCTCCCAAC TGGTGATTCCAGAGACGACT 55.0 50.0 58.1 58.1 936-1496 bp 1.0 90.8
trnE-UUC-trnT-GGU_p2 CTGACTATCGTGCTCCCAAC TGGTACATGAAGCTGCGATC 55.0 50.0 58.1 58.1 1355-1915 bp 1.0 90.7
trnE-UUC-trnT-GGU_p3 GAAGAAGAGCCCATTCGGAG TGGTACATGAAGCTGCGATC 55.0 50.0 58.1 58.1 1102 bp 0.052632 41.2
trnE-UUC-trnT-GGU_p4 GAAGAAGAGCCCATTCGGAG TGGTGATTCCAGAGACGACT 55.0 50.0 58.1 58.1 681 bp 0.052632 41.1
trnE-UUC-trnT-GGU_p5 GAAGAAGAGCCCATTCGGAG TACCGCTGAGTTAAAAGGGC 55.0 50.0 58.1 57.9 390 bp 0.052632 40.9