Hotspot

petN-psbM

LSC · 28725-29719 · 995 bp

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Pi 0.0265
MarkerSeek score 67.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
995
variable_sites
94
pi
0.0265
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AAGACCGGAAGAGCCCTTAT AAGTACCCCCTTCTGACTCC 50.0 55.0 58.1 58.0 1759-2047 bp 1.0 88.3
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT AAGTACCCCCTTCTGACTCC 50.0 55.0 58.2 58.0 1371-1636 bp 1.0 87.8
petN-psbM_p3 GAAGGGCTATCACGACTTCC AAGTACCCCCTTCTGACTCC 55.0 55.0 57.8 58.0 1978-2173 bp 0.947368 82.9
petN-psbM_p4 AGTCCCTTTTTGACTCTGCG AAGTACCCCCTTCTGACTCC 50.0 55.0 58.1 58.0 1469-1734 bp 0.684211 73.1
petN-psbM_p5 CAACGGGCAAAAAGAACAGG AAGTACCCCCTTCTGACTCC 50.0 55.0 58.1 58.0 1602-1839 bp 0.473684 65.5