Hotspot

trnC-GCA-petN

LSC · 28000-28634 · 635 bp

Back to Daucus

Pi 0.0268
MarkerSeek score 69.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
635
variable_sites
56
pi
0.0268
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 691-750 bp 1.0 87.1
trnC-GCA-petN_p2 CGATATGGCCGAGTGGTAAG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.9 58.2 731-790 bp 1.0 86.8
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 752-811 bp 1.0 84.8
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 709-768 bp 1.0 84.7
trnC-GCA-petN_p5 CGATATGGCCGAGTGGTAAG AGAGTCCACTTCTTCCCCAT 55.0 50.0 57.9 57.6 792-851 bp 1.0 84.6