Hotspot

rpoB-trnC-GCA

LSC · 26695-27928 · 1234 bp

Back to Daucus

Pi 0.0213
MarkerSeek score 64.90
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1234
variable_sites
100
pi
0.0213
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CTTCCATCAAGCCCTGATCC CTTACCACTCGGCCATATCG 55.0 55.0 58.0 57.9 1323-1356 bp 1.0 89.6
rpoB-trnC-GCA_p2 CTTCCATCAAGCCCTGATCC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.0 58.1 1363-1396 bp 1.0 89.3
rpoB-trnC-GCA_p3 CCGCCTACACAAGCAAATTG CTTACCACTCGGCCATATCG 50.0 55.0 58.0 57.9 1927-1960 bp 1.0 88.5
rpoB-trnC-GCA_p4 CCGCCTACACAAGCAAATTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1967-2000 bp 1.0 88.3
rpoB-trnC-GCA_p5 CCGCCTACACAAGCAAATTG ATAAGGGCTCTTCCGGTCTT 50.0 50.0 58.0 58.1 2276-2310 bp 1.0 87.8