Hotspot

trnS-GCU-trnG-TCC

LSC · 8517-9010 · 494 bp

Back to Daucus

Pi 0.0263
MarkerSeek score 67.20
Primer available yes
Label trnS-GCU-trnG-TCC

Feature Metadata

label_name
trnS-GCU-trnG-TCC
feature_type
igs
region
LSC
length_bp
494
variable_sites
47
pi
0.0263
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-TCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 549-639 bp 1.0 61.9
trnS-GCU-trnG-TCC_p2 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 598-688 bp 1.0 60.2
trnS-GCU-trnG-TCC_p3 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 550-640 bp 1.0 59.9
trnS-GCU-trnG-TCC_p4 ACGGAAAGAGAGGGATTCGA AACGAATCACACTTTTACCACT 50.0 36.4 58.2 56.2 599-689 bp 1.0 58.2
trnS-GCU-trnG-TCC_p5 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 535-625 bp 0.947368 55.9