Hotspot

rps16-trnQ-UUG

LSC · 5927-7163 · 1237 bp

Back to Daucus

Pi 0.0231
MarkerSeek score 68.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1237
variable_sites
109
pi
0.0231
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1016-1587 bp 1.0 88.3
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1017-1588 bp 1.0 88.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1027-1598 bp 1.0 87.5
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 980-1551 bp 1.0 85.8
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1041-1612 bp 1.0 85.6