Hotspot

trnK-UUU-rps16

LSC · 4086-4817 · 732 bp

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Pi 0.0286
MarkerSeek score 70.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
732
variable_sites
81
pi
0.0286
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG GATTCAAAGTGTATCGCGCG 50.0 50.0 58.0 58.0 1536-1578 bp 0.947368 87.6
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1589-1631 bp 0.947368 87.5
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1588-1630 bp 0.947368 87.5
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1588-1630 bp 0.947368 87.5
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1587-1629 bp 0.947368 87.5