Hotspot

ycf1

SSC · 125881-131508 · 5628 bp

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Pi 0.00628
MarkerSeek score 41.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5628
variable_sites
53
pi
0.00628
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CAGGTTTCTGCTAGCGCTAT CTCAAAGGCGCAAAACTGTT 50.0 45.0 57.8 57.8 271 bp 1.0 78.3
ycf1_p2 GCTATCCGTGCTTGTTCCTT CTCAAAGGCGCAAAACTGTT 50.0 45.0 58.3 57.8 256 bp 1.0 78.1
ycf1_p3 TCCGTGCTTGTTCCTTTCTT CTCAAAGGCGCAAAACTGTT 45.0 45.0 57.7 57.8 252 bp 1.0 77.5
ycf1_p4 GGTTTCTGCTAGCGCTATCC CTCAAAGGCGCAAAACTGTT 55.0 45.0 58.5 57.8 269 bp 1.0 76.1
ycf1_p5 GCGCCAAAGGAATGTTATCC CTCAAAGGCGCAAAACTGTT 50.0 45.0 57.5 57.8 291 bp 1.0 76.0