Hotspot

ndhF

SSC · 112065-114287 · 2223 bp

Pi 0.006

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 20
pi: 0.006
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TGAATCCCCGTTCGATTCAC	50.0	50.0	58.0	58.0	2796-2802 bp	1.0	80.3
ndhF_p2	ACAGGAACAAGAGGGATCCA	AATCCCCGTTCGATTCACTG	50.0	50.0	58.0	58.0	2794-2800 bp	1.0	80.3
ndhF_p3	ACTGCTTCCGAAATGAAGGG	TGAATCCCCGTTCGATTCAC	50.0	50.0	58.2	58.0	2822-2828 bp	1.0	79.3
ndhF_p4	ACTGCTTCCGAAATGAAGGG	AATCCCCGTTCGATTCACTG	50.0	50.0	58.2	58.0	2820-2826 bp	1.0	79.2
ndhF_p5	TAGGCAGAATACCCTCACCC	TGAATCCCCGTTCGATTCAC	55.0	50.0	58.2	58.0	2902-2908 bp	1.0	79.1