Hotspot

ndhF-rpl32

SSC · 114288-115076 · 789 bp

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Pi 0.00591
MarkerSeek score 53.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
789
variable_sites
7
pi
0.00591
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAAGAATAAGTCCCGCCCC TGATGTCGAAGTGCGTTTTT 50.0 40.0 57.9 56.6 902-909 bp 1.0 68.8
ndhF-rpl32_p2 AAAAGAATAAGTCCCGCCCC TTGATGTCGAAGTGCGTTTT 50.0 40.0 57.9 56.6 903-910 bp 1.0 68.8
ndhF-rpl32_p3 AAAAGAATAAGTCCCGCCCC TTTGATGTCGAAGTGCGTTT 50.0 40.0 57.9 56.6 904-911 bp 1.0 68.8
ndhF-rpl32_p4 AAAAGAATAAGTCCCGCCCC TTTTGATGTCGAAGTGCGTT 50.0 40.0 57.9 56.6 905-912 bp 1.0 68.8
ndhF-rpl32_p5 AAAAGAATAAGTCCCGCCCC TTTTTGATGTCGAAGTGCGT 50.0 40.0 57.9 56.6 906-913 bp 1.0 68.8