Hotspot

rps11-rpl36

LSC · 81738-81838 · 101 bp

Back to Datura

Pi 0.0132
MarkerSeek score 60.90
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
101
variable_sites
2
pi
0.0132
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 AATACGTCCATTCCTACGCG ATTTGTTCCAACCCGAGACA 50.0 45.0 57.9 57.4 188 bp 1.0 74.6
rps11-rpl36_p2 AATACGTCCATTCCTACGCG TTTGTTCCAACCCGAGACAT 50.0 45.0 57.9 57.4 187 bp 1.0 74.6
rps11-rpl36_p3 ATTCTACGTGCACCCTTACG ATTTGTTCCAACCCGAGACA 50.0 45.0 57.7 57.4 214 bp 1.0 73.2
rps11-rpl36_p4 ATTCTACGTGCACCCTTACG TTTGTTCCAACCCGAGACAT 50.0 45.0 57.7 57.4 213 bp 1.0 73.2
rps11-rpl36_p5 TTGGTATTCTACGTGCACCC TTTGTTCCAACCCGAGACAT 50.0 45.0 57.6 57.4 218 bp 1.0 72.4