Hotspot

petA-psbJ

LSC · 65226-66176 · 951 bp

Back to Datura

Pi 0.00631
MarkerSeek score 63.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
951
variable_sites
9
pi
0.00631
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1005-1011 bp 1.0 77.1
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1007-1013 bp 1.0 77.1
petA-psbJ_p3 AGAAGGTTCAATTGGCCGAA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.7 57.6 1004-1010 bp 1.0 76.2
petA-psbJ_p4 ACGTGTCCAAGGACTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.6 57.6 1084-1090 bp 1.0 75.1
petA-psbJ_p5 TCCATTACGTGTCCAAGGAC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.2 57.6 1090-1096 bp 1.0 72.4