Hotspot

ndhC-trnV-UAC

LSC · 52260-53353 · 1094 bp

Back to Datura

Pi 0.0067
MarkerSeek score 63.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1094
variable_sites
11
pi
0.0067
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTGCTAATCGGGGCTAACAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1232-1241 bp 1.0 79.3
ndhC-trnV-UAC_p2 GCTAATCGGGGCTAACACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1230-1239 bp 1.0 78.5
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1235-1244 bp 1.0 76.8
ndhC-trnV-UAC_p4 TTGCTAATCGGGGCTAACAC GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1231-1240 bp 1.0 76.6
ndhC-trnV-UAC_p5 GGCTAACACTCCGGAAATGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1221-1230 bp 1.0 76.4