Hotspot

petN-psbM

LSC · 29476-30592 · 1117 bp

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Pi 0.00477
MarkerSeek score 61.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1117
variable_sites
8
pi
0.00477
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA AGTTCCTACCGCTTTTCTACT 45.0 42.9 58.0 56.4 1251-1253 bp 1.0 59.6
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG AGTTCCTACCGCTTTTCTACT 50.0 42.9 57.8 56.4 1257-1259 bp 1.0 58.1
petN-psbM_p3 GGGGAAGGAGTGGACTCTAG AGTTCCTACCGCTTTTCTACT 60.0 42.9 58.2 56.4 1195-1197 bp 1.0 57.9
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA AGTTCCTACCGCTTTTCTACTT 45.0 40.9 58.0 57.1 1251-1253 bp 1.0 57.4
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA TCTAGTTCCTACCGCTTTTCT 45.0 42.9 58.0 56.1 1254-1256 bp 1.0 57.3