Hotspot

atpH-atpI

LSC · 13899-15091 · 1193 bp

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Pi 0.00726
MarkerSeek score 63.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1193
variable_sites
13
pi
0.00726
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TACCTGTCATGCTCCTTGGA 45.0 50.0 58.0 58.0 1329-1330 bp 1.0 80.1
atpH-atpI_p2 TACCCTCTACAGCTTGACCC TACCTGTCATGCTCCTTGGA 55.0 50.0 58.1 58.0 1412-1413 bp 1.0 79.3
atpH-atpI_p3 GCGATACCCTCTACAGCTTG TACCTGTCATGCTCCTTGGA 55.0 50.0 57.9 58.0 1416-1417 bp 1.0 79.3
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA TACCTGTCATGCTCCTTGGA 45.0 50.0 58.2 58.0 1330-1331 bp 1.0 79.0
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TACCTGTCATGCTCCTTGGA 50.0 50.0 57.8 58.0 1344-1345 bp 1.0 78.7