Hotspot

atpF

LSC · 32621-34007 · 1387 bp

Back to Dasypyrum

Pi 0.00218

MarkerSeek score 60.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1387
variable_sites: 3
pi: 0.00218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1518-1529 bp	1.0	79.4
atpF_p2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	45.0	45.0	58.1	57.9	1519-1530 bp	1.0	79.4
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	45.0	45.0	58.1	57.9	1520-1531 bp	1.0	79.4
atpF_p4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	45.0	45.0	58.1	57.9	1521-1532 bp	1.0	79.4
atpF_p5	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTAGTG	45.0	50.0	58.1	57.9	1553-1564 bp	1.0	79.1