Hotspot

psbE-petL

LSC · 62487-63653 · 1167 bp

Back to Dasypyrum

Pi 0.00686

MarkerSeek score 62.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 8
pi: 0.00686
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	45.0	50.0	58.0	58.3	1371 bp	1.0	78.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	50.0	50.0	57.9	58.3	1360 bp	1.0	78.0
psbE-petL_p3	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1325 bp	1.0	76.6
psbE-petL_p4	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1323 bp	1.0	76.6
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	50.0	50.0	58.2	58.3	1362 bp	1.0	76.5