Hotspot

ndhC-trnV-UAC

LSC · 49781-50685 · 905 bp

Back to Dasypyrum

Pi 0.00444

MarkerSeek score 61.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 4
pi: 0.00444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCGAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.1	57.9	1010-1015 bp	1.0	79.5
ndhC-trnV-UAC_p2	GCCCTGAAATCGAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.1	58.3	1011-1016 bp	1.0	77.7
ndhC-trnV-UAC_p3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.3	57.9	1039-1044 bp	1.0	74.8
ndhC-trnV-UAC_p4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.7	57.9	1038-1043 bp	1.0	74.2
ndhC-trnV-UAC_p5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	1029-1034 bp	1.0	73.9