Hotspot

trnG-UCC-trnM-CAU

LSC · 13536-14659 · 1124 bp

Back to Dasypyrum

Pi 0.00488
MarkerSeek score 57.30
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1124
variable_sites
5
pi
0.00488
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1208-1248 bp 1.0 45.1
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1204-1244 bp 1.0 44.3
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1205-1245 bp 1.0 44.3
trnG-UCC-trnM-CAU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1211-1251 bp 1.0 44.3
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1210-1250 bp 1.0 44.3